Review



pcmvdtx2 myc flag  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    OriGene pcmvdtx2 myc flag
    Pcmvdtx2 Myc Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/DTX2+(NM_020892)+Human+Tagged+ORF+Clone/pm42229860-66-34-42
    Average 94 stars, based on 1 article reviews
    pcmvdtx2 myc flag - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Unveiling an Arpp-19 phosphorylation switch that grants chromosome stability.
    Article Snippet: .. Mammalian expression plasmid for Myc-Flag-tagged-human-Arpp-19 (WT-Arpp-19) was purchased from OriGene (Cat# RC210574). .. To generate S23A-Arpp-19 and S23D-Arpp-19 mutant versions, serine 23 of human Arpp-19 was mutagenized into alanine (AGT → GAT) and aspartate (AGT→ GCT), respectively, by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) using the MycFlag-WT-Arpp-19 construct as template.

    Plasmid Preparation:

    Article Title: Unveiling an Arpp-19 phosphorylation switch that grants chromosome stability.
    Article Snippet: .. Mammalian expression plasmid for Myc-Flag-tagged-human-Arpp-19 (WT-Arpp-19) was purchased from OriGene (Cat# RC210574). .. To generate S23A-Arpp-19 and S23D-Arpp-19 mutant versions, serine 23 of human Arpp-19 was mutagenized into alanine (AGT → GAT) and aspartate (AGT→ GCT), respectively, by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) using the MycFlag-WT-Arpp-19 construct as template.

    Article Title: Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity
    Article Snippet: .. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235); Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT→GCT) and serine 684 into alanine (2050–2052 nt TCT→GCT) by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using Kif18A-WT as template. .. Custom siRNAs targeting the 3 ’ -UTR of human WEE1 (#1: 5′-CUG UAA ACU UGU AGC AUU AUU-3’; #2: 5′-GUA CAU AGC UGU UUG AAA UUU-3’; #3: 5′-GGG CUU UAU UAC AGA CAU AUU-3′) were designed using siDESIGN Center tool by Horizon and purchased from Dharmacon.

    Article Title: Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis.
    Article Snippet: In this study, we utilized enzyme-catalyzed proximity labeling with the engineered promiscuous biotin ligase Turbo-ID to identify the proxisome of the ROMK2 channel.. This channel resides in various cellular membrane compartments of the cell including the plasma membrane, endoplasmic reticulum and mitochondria.. Within mitochondria, ROMK2 has been suggested as a pore-forming subunit of mitochondrial ATP-regulated potassium channel (mitoKATP).

    Article Title: Chromosome alignment relies on spindle-localized control of Cdk1 activity
    Article Snippet: .. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235); Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT◊GCT) and serine 684 into alanine (2050–2052 nt TCT◊GCT) by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using Kif18A-WT as template. .. Custom siRNAs targeting the 3’-UTR of human WEE1 (#1: 5’-CUGUAAACUUGUAGCAUUAUU-3’; #2: 5’- GUACAUAGCUGUUUGAAAUUU-3’; #3: 5’-GGGCUUUAUUACAGACAUAUU-3’) were designed using siDESIGN Center tool by Horizon and purchased from Dharmacon.

    Mutagenesis:

    Article Title: Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity
    Article Snippet: .. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235); Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT→GCT) and serine 684 into alanine (2050–2052 nt TCT→GCT) by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using Kif18A-WT as template. .. Custom siRNAs targeting the 3 ’ -UTR of human WEE1 (#1: 5′-CUG UAA ACU UGU AGC AUU AUU-3’; #2: 5′-GUA CAU AGC UGU UUG AAA UUU-3’; #3: 5′-GGG CUU UAU UAC AGA CAU AUU-3′) were designed using siDESIGN Center tool by Horizon and purchased from Dharmacon.

    Article Title: Chromosome alignment relies on spindle-localized control of Cdk1 activity
    Article Snippet: .. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235); Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT◊GCT) and serine 684 into alanine (2050–2052 nt TCT◊GCT) by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using Kif18A-WT as template. .. Custom siRNAs targeting the 3’-UTR of human WEE1 (#1: 5’-CUGUAAACUUGUAGCAUUAUU-3’; #2: 5’- GUACAUAGCUGUUUGAAAUUU-3’; #3: 5’-GGGCUUUAUUACAGACAUAUU-3’) were designed using siDESIGN Center tool by Horizon and purchased from Dharmacon.

    Generated:

    Article Title: Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity
    Article Snippet: .. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235); Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT→GCT) and serine 684 into alanine (2050–2052 nt TCT→GCT) by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using Kif18A-WT as template. .. Custom siRNAs targeting the 3 ’ -UTR of human WEE1 (#1: 5′-CUG UAA ACU UGU AGC AUU AUU-3’; #2: 5′-GUA CAU AGC UGU UUG AAA UUU-3’; #3: 5′-GGG CUU UAU UAC AGA CAU AUU-3′) were designed using siDESIGN Center tool by Horizon and purchased from Dharmacon.

    Article Title: Chromosome alignment relies on spindle-localized control of Cdk1 activity
    Article Snippet: .. Myc-Flag-tagged-human-Kif18A-WT (Kif18A-WT) plasmid was purchased from Origene (Cat# RC208235); Kif18A-AA mutant version was generated via mutagenesis: serine 674 was mutagenized into alanine (2020–2022 nt TCT◊GCT) and serine 684 into alanine (2050–2052 nt TCT◊GCT) by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using Kif18A-WT as template. .. Custom siRNAs targeting the 3’-UTR of human WEE1 (#1: 5’-CUGUAAACUUGUAGCAUUAUU-3’; #2: 5’- GUACAUAGCUGUUUGAAAUUU-3’; #3: 5’-GGGCUUUAUUACAGACAUAUU-3’) were designed using siDESIGN Center tool by Horizon and purchased from Dharmacon.

    FLAG-tag:

    Article Title: Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis.
    Article Snippet: In this study, we utilized enzyme-catalyzed proximity labeling with the engineered promiscuous biotin ligase Turbo-ID to identify the proxisome of the ROMK2 channel.. This channel resides in various cellular membrane compartments of the cell including the plasma membrane, endoplasmic reticulum and mitochondria.. Within mitochondria, ROMK2 has been suggested as a pore-forming subunit of mitochondrial ATP-regulated potassium channel (mitoKATP).

    Co-Immunoprecipitation Assay:

    Article Title: Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis.
    Article Snippet: In this study, we utilized enzyme-catalyzed proximity labeling with the engineered promiscuous biotin ligase Turbo-ID to identify the proxisome of the ROMK2 channel.. This channel resides in various cellular membrane compartments of the cell including the plasma membrane, endoplasmic reticulum and mitochondria.. Within mitochondria, ROMK2 has been suggested as a pore-forming subunit of mitochondrial ATP-regulated potassium channel (mitoKATP).

    Clone Assay:

    Article Title: Coordination of autophagosome closure and release by the Alzheimer's disease-associated protein BIN1.
    Article Snippet: 47 Neurons were allowed to mature for 7 days after induction in Neurobasal-A media (Thermo Fisher #10888022) supplemented with B-27 (Thermo Fisher #17504044) on Geltrex-coated plates (Thermo Fisher #A1413302) at 37 ◦ C in 5% CO 2 prior to use in experiments. .. Myc-FLAG-tagged human BIN1 isoform 9 was cloned from Myc-FLAG-tagged human BIN1 isoform 8 (Origene #RC20616) by removing the PI-interacting motif. .. A QuikChange XL Site-Directed Mutagenesis kit (Agilent #200516) was used following the manufacturer’s instructions and the plasmid identity was confirmed by Sanger sequencing.

    Modification:

    Article Title: p21 produces a bioactive secretome that places stressed cells under immunosurveillance
    Article Snippet: .. The Myc-Flag-tagged cDNA for mouse p21 was obtained from Origene (#MR227529) and subcloned into the lentiviral pTSIN - PGK-puro2 backbone ( 43 , 66 ) or dox-inducible pTRIPZ-PKG-puro backbone (modified from GE Dharmacon) ( 67 ). .. Similarly, the Myc-Flag-tagged cDNAs for mouse p16 (Origene, #MR227284) and mouse p27 (Origene, #MR201957) were also subcloned into the lentiviral pTSIN - PGK-puro2 backbone.



    Similar Products

    c myc flag protein  (MedChemExpress)
    94
    MedChemExpress c myc flag protein
    C Myc Flag Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/MYC%2C+Human/pm42028950-109-1-9
    Average 94 stars, based on 1 article reviews
    c myc flag protein - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    pcmvdtx2 myc flag  (OriGene)
    94
    OriGene pcmvdtx2 myc flag
    Pcmvdtx2 Myc Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/DTX2+(NM_020892)+Human+Tagged+ORF+Clone/pm42229860-66-34-42
    Average 94 stars, based on 1 article reviews
    pcmvdtx2 myc flag - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    cdc37 myc flag  (OriGene)
    94
    OriGene cdc37 myc flag
    Cdc37 Myc Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/CDC37+(NM_007065)+Human+Tagged+ORF+Clone/bio_rxiv__64898__2026__05__08__723844-161-0-4
    Average 94 stars, based on 1 article reviews
    cdc37 myc flag - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    anti flag  (Proteintech)
    96
    Proteintech anti flag
    Anti Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/MYC+tag+Antibody/pm41922343-323-51-53
    Average 96 stars, based on 1 article reviews
    anti flag - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    antibodies against flag  (Proteintech)
    96
    Proteintech antibodies against flag
    USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
    Antibodies Against Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/MYC+tag+Antibody/pmc13015209-54-0-60
    Average 96 stars, based on 1 article reviews
    antibodies against flag - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti flag monoclonal antibody  (Proteintech)
    96
    Proteintech anti flag monoclonal antibody
    USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
    Anti Flag Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/MYC+tag+Antibody/pm41910148-174-51-54
    Average 96 stars, based on 1 article reviews
    anti flag monoclonal antibody - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    anti flag magnetic beads  (MedChemExpress)
    97
    MedChemExpress anti flag magnetic beads
    USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
    Anti Flag Magnetic Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/Anti-c-Myc+Magnetic+Beads/pm41910270-254-80-83
    Average 97 stars, based on 1 article reviews
    anti flag magnetic beads - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    pcmv a20 myc flag  (OriGene)
    94
    OriGene pcmv a20 myc flag
    USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
    Pcmv A20 Myc Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/TNFAIP3+(NM_006290)+Human+Tagged+ORF+Clone/pmc13153315-243-6-8
    Average 94 stars, based on 1 article reviews
    pcmv a20 myc flag - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    pcmv6 g1 atp6 myc flag  (Addgene inc)
    93
    Addgene inc pcmv6 g1 atp6 myc flag
    USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with <t>FLAG-USP25</t> and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with <t>anti-FLAG</t> (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.
    Pcmv6 G1 Atp6 Myc Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+flag/pCMV6-G1-ATP6-Myc-FLAG+(puromycin)+(Plasmid+%2386851)/pmc12925242-74-0-2
    Average 93 stars, based on 1 article reviews
    pcmv6 g1 atp6 myc flag - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with FLAG-USP25 and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with anti-FLAG (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.

    Journal: eBioMedicine

    Article Title: USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses

    doi: 10.1016/j.ebiom.2026.106213

    Figure Lengend Snippet: USP25 interacts with RIPK1 through the USP domain. (A) Schematic diagram of the proteomic screening for USP25-interacting proteins. (B) Two-dimensional plot of USP25-binding proteins, with the Y axis showing protein intensity and the X axis showing protein molecular weight. (C) List of potential substrates with unique peptides ≥2 identified by the mass spectrometry analysis. (D–E) Whole cell lysates of BMDMs were immunoprecipitated with anti-USP25 (D) or anti-RIPK1 (E) antibodies, followed by Western blot analysis. Rabbit IgG served as negative control. (F–G) HEK293 cells were co-transfected with FLAG-USP25 and MYC-RIPK1 plasmids for 24 h. Whole-cell lysates were immunoprecipitated with anti-FLAG (F) or anti-MYC (G) antibodies, followed by Western blot analysis. (H) BMDMs were left untreated or treated with ox-LDL (50 μg/mL) for 30 min before lysis. Whole-cell lysates were immunoprecipitated with anti-USP25 antibody, followed by Western blot analysis. (I) Subcellular distribution of USP25 (red) and RIPK1 (green) in BMDMs treated with or without ox-LDL (50 μg/mL) for 30 min was detected by immunofluorescence. Scale bar: 5 μm. (J) Schematic diagram of structural domains and truncation mutants of USP25. (K) HEK293 cells were co-transfected with indicated plasmids for 24 h. Cell lysates were immunoprecipitated with anti-FLAG antibody and further analysed by Western blot with indicated antibodies.

    Article Snippet: Antibodies against FLAG (Cat#: 20543-1-AP, 1: 1000, RRID: AB_11232216 ), MYC (Cat#: 60003-2-Ig, 1: 1000, RRID: AB_2734122 ), HA (Cat#: 51064-2-AP, 1: 1000, RRID: AB_11042321 ), CD36 (Cat#: 18836-1-AP, 1:1000, RRID: AB_10597244 ), ASK1 (Cat#: 28201-1-AP, 1: 1000, RRID: AB_2782957 ), MLKL (Cat#: 66675-1-Ig, 1:1000, RRID: AB_2882029 ), and p-ASK1 (Cat#: 28846-1-AP, 1: 1000, RRID: AB_2881220 ) were purchased from Proteintech (Wuhan, China).

    Techniques: Binding Assay, Molecular Weight, Mass Spectrometry, Immunoprecipitation, Western Blot, Negative Control, Transfection, Lysis, Immunofluorescence